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The
Loopamp®
Realtime Turbidimeter (LA-320C) is a device specifically designed for
real-time reporting of the LAMP (Loopmediated
Isothermal Amplification) reaction, which utilizes a novel gene amplification method
developed by EIKEN CHEMICAL CO., LTD. User-friendly software controls
the turbidimeter from a computer, allowing temperature and reaction
time modifications for different tests. The positive results are
monitored through detection of the white turbidity formed by the
amplification by-product, magnesium pyrophosphate.
Features of LA-320C
• Up to 32 samples can be
amplified and monitored every six seconds simultaneously.
• Four heat blocks, each
holding eight samples, can be set individually with different
temperature and reaction time, allowing up to four different tests to
be performed at the same time.
• The lid of the
turbidimeter is heated, preventing samples from vaporizing and
condensing on the tube cover during the reaction.
• The user-friendly
software is very simple to operate.
• As with other real-time
amplification methods, the LAMP reaction positive reporting time is
related to the initial copies of the template. With the reporting
function of the Loopamp® Realtime Turbidimeter and a set of standard
template samples, the LAMP amplification can be used to quantify the
template in the samples.
Amplification
Reaction and Detection Principle
The
Loopamp®
Realtime Turbidimeter (LA-320C) controls the reaction temperature and
time for the LAMP reaction. The four reaction blocks can be
individually controlled. Its optical system detects the white
turbidity caused by the amplification by-product (magnesium
pyrophosphate) every six seconds. The software records and
reports the result in real-time. At the end of the reaction, the
reaction blocks are programmed to heat and inactivate the enzyme to
stop the reaction.
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LA-320C uses Windows®
applicable user interface which
allows simple operation.
•Ability to input various
parameters such as reaction time, temperature, recording and
reporting time, etc.
•Visualization of a real-time
amplification curve
•Adjustable
baselines
•Ability to export
data as
CSV files.
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Main Specifications
| Reaction Block: |
8 turbidity channels with each
heating block, 4 blocks (32 samples) |
| Temperature control: |
Block: 55-70°C ± 0.5°C (for
enzyme inactivation 65 - 80°C) |
| Hot Bonnet: 65 - 90°C |
| Dimensions: |
W245mm x D282mm x H188mm (projected
parts excluded) |
| Weight: |
6.5 kg |
| Power supply: |
A/C 120V |
| Operation Temperature: |
10 - 30°C |
| PC: |
Microsoft Windows® 2000 or XP
compatible |
Inquiries
For more information about this device, LAMP method or Loopamp® products, please send an email to sas@sascientific.com
or call 1-800-272-2710.
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