•Rapid
and specific detection of Escherichia coli O157 in foods
The Loopamp® Escherichia
coli O157 Detection Kit uses sequences related to Escherichia coli
serotype O157 as the targeting template. By using four primers to
recognize six distinct regions in the gene, this test is highly
specific. Due to the high amplification efficiency of LAMP, the
results are achieved within one hour. Including pre-enrichment
culture and DNA purification, testing of Escherichia coli O157
in foods
has a turnaround time of 24 hours using this test. No further
enrichment culture is needed.
•Amplification and
detection in one single- tube with real-time results.
With Loopamp® Realtime
Turbidimeters, amplification and real-time detection are achieved in
one step.
Since electrophoresis is not required, the entire process for each type is
completed within one reaction tube, which greatly reduces the possibility of
cross-contamination frequently associated with nucleic acid
amplification technologies.
•Alternative
method of detection
Because LAMP is an isothermal reaction, the testing can
also be performed in a water bath or a heat block set at 65°C.
The endpoint results can be visualized by adding Loopamp® Fluorescent
Detection Reagent (sold separately) to the initial master mix.
• For more detailed information, view
the Package Insert
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