Rapid and specific detection of Verotoxin-producing Escherichia coli Detection Kit

Loopamp® Verotoxin-producing Escherichia coli Detection Kit uses the Verotoxin (VT, also known as Shiga toxin or Shiga-like toxin) genes as the target sequences. By using four primers to recognize six distinct regions in the genes, this test is highly specific. Due to the high amplification efficiency of LAMP, the results are achieved within one hour. Including pre-enrichment culture and DNA purification, testing of Verotoxin-producing Escherichia coli in foods has a turnaround time of 24 hours using this test. No further enrichment culture is needed.

Amplification and detection in one single-tube with real-time results

With Loopamp® Realtime Turbidimeters, amplification and real-time detection are achieved in one step. Since electrophoresis is not required, the entire process is completed within one reaction tube, which greatly reduces the possibility of cross-contamination frequently associated with nucleic acid amplification technologies.

Alternative method of detection

Because LAMP is an isothermal reaction, the test can also be performed in a water bath or a heat block set at 65°C. The endpoint results can be visualized by adding Loopamp® Fluorescent Detection Reagent (sold separately) to the initial master mix.