Rapid and specific typing of Verotoxin I (VT1) and II (VT2) produced by Verotoxin producing Escherichia coli

Loopamp® Verotoxin Typing Kit uses Verotoxin (VT1 and VT2, also known as Shiga toxins Stx1 and Stx2, or Shiga-like toxins SLT1 and SLT2) genes as the target sequences. By using four primers to recognize six distinct regions in each gene, these tests are highly specific. Due to the high amplification efficiency of LAMP, determination of Verotoxin type is completed within one hour after a 10 minute DNA isolation from microbial culture or colonies.

Amplification and detection in one single-tube with real-time results.

With Loopamp® Realtime Turbidimeters, amplification and real-time detection are achieved in one step. Since electrophoresis is not required, the entire process for each type is completed within one reaction tube, which greatly reduces the possibility of cross-contamination frequently associated with nucleic acid amplification technologies.

Alternative method of detection

Because LAMP is an isothermal reaction, the testing can also be performed in a water bath or a heat block set at 65°C. The endpoint results can be visualized by adding Loopamp® Fluorescent Detection Reagent (sold separately) to the initial master mix.