Visual detection of LAMP amplification

The Loopamp® Fluorescent Detection Reagent is added into the reaction tube during the reaction setup. The LAMP amplification is then performed in a constant temperature (60-65°C) in a heat block or a water bath for the reaction time required by a specific primer set. The enzyme is inactivated by heating the tubes at 80-95°C for 2 minutes. Presence of fluorescence under a UV light (254-366 nm) indicates a positive result.

Turbidity detection will not be affected

The presence of this reagent will not affect the result by real-time turbidity detection (standard detection method) using a Loopamp® Realtime Turbidimeter.

No extra effort to confirm the amplification

Amplification results can be visually observed through the presence of fluorescence. Because confirmation of results with electrophoresis, which requires the opening of the reaction tubes, is not required, the possibility of cross-contamination frequently associated with DNA/RNA amplification technologies is greatly reduced.