Salmonella spp. Detection Kit
Rapid and specific detection of Salmonella spp. in foods
The SAS™ Salmonella spp. Detection Kit uses the Salmonella invasion gene (invA) as the target sequence. By using four primers to recognize six distinct regions in invA gene, this test is highly specific. Due to the high amplification efficiency of isothermal amplification technology (Loopamp®), the results are achieved within one hour. Including pre-enrichment culture and DNA purification, testing of Salmonella spp. in foods has a turnaround time of 24 hours using this test. No further enrichment culture is needed.
Amplification and detection in a single tube with real-time results
With Realtime Turbidimeters, amplification and real-time detection are achieved in one step. Since electrophoresis is not required, the entire process is completed within one reaction tube, which greatly reduces the possibility of cross-contamination frequently associated with nucleic acid amplification technologies.
Alternative method of detection
Because this is an isothermal reaction, the test can also be performed in a water bath or a heat block set at 65°C. The endpoint results can be visualized by adding Fluorescent Detection Reagent (sold separately) to the initial master mix.
The SAS™ Salmonella spp. Detection kit is now AOAC approved.
Target: Salmonella spp.
Matrices: Ground Poultry, Ground Beef, Beef Trim, Chicken Carcass, Lettuce, and Spinach
Enrichment medium: SAS™ Enrichment media
Enrichment time: 6-7 hours for 25g Ground Poultry, 25g Ground Beef, 200g Spinach, 200g Lettuce, and 400mL Chicken Carcass rinsate. 16-20 hours for 375g composited Ground Beef and 375g Beef Trim.
Amplification time: 60 minutes
Instrument: LA-500 Realtime Turbidimeter
Detection: ~1cfu/test sample